Description du livre
The authoritative guide on protein purification—now completely updated and revised
Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.
Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:
High-resolution reversed-phase liquid chromatography
Electrophoresis in gels
Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients
Affinity ligands from chemical and biological combinatorial libraries
Membrane separations
Refolding of inclusion body proteins from E. coli
Purification of PEGylated proteins
High throughput screening techniques in protein purification
The history of protein chromatography